HDAC9 Antibody (3L5E5)

Novus Biologicals | Catalog # NBP3-16097

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3L5E5 expressed in HEK293
View all HDAC9 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC9 (Q9UKV0). MHSMISSVDVKSEVPVGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKELLAIKQQQELL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC9 Antibody (3L5E5)

Western Blot: HDAC9 Antibody (3L5E5) [NBP3-16097]

Western Blot: HDAC9 Antibody (3L5E5) [NBP3-16097]

Western Blot: HDAC9 Antibody (3L5E5) [NBP3-16097] - Western blot analysis of extracts of various cell lines, using HDAC9 Rabbit mAb (NBP3-16097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097]

Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097]

Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097] - Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC9 Rabbit mAb (NBP3-16097) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097]

Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097]

Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097] - Immunohistochemistry of paraffin-embedded rat testis using HDAC9 Rabbit mAb (NBP3-16097) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
HDAC9 Antibody (3L5E5)

Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] -

Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] - Confocal imaging of U-2 OS cells using HDAC9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
HDAC9 Antibody (3L5E5)

Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] -

Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] - Confocal imaging of K-562 cells using HDAC9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for HDAC9 Antibody (3L5E5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HDAC9

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Long Name

Histone deacetylase 9

Alternate Names

HD7b, HD9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR

Gene Symbol

HDAC9

Additional HDAC9 Products

Product Documents for HDAC9 Antibody (3L5E5)

Certificate of Analysis

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Product Specific Notices for HDAC9 Antibody (3L5E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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