HDLBP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83287

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDLBP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDLBP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDLBP Antibody [NBP1-83287]

Immunocytochemistry/ Immunofluorescence: HDLBP Antibody [NBP1-83287]

Immunocytochemistry/Immunofluorescence: HDLBP Antibody [NBP1-83287] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: HDLBP Antibody [NBP1-83287]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP1-83287]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP1-83287] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for HDLBP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDLBP

HDL-binding protein 1 (HDLBP) is a novel protein that may be involved in the regulation of the delivery of fats to cells for energy and storage. Digested fats travel to the small intestine, where they are packaged into chylomicrons (particles filled with triglycerides). Chylomicrons then travel through the bloodstream and deliver triglycerides to tissues that are hungry for fuel or to adipose tissue for energy storage. Triglycerides are broken down or hydrolyzed by the enzyme lipoprotein lipase (LpL). The triglyceride breakdown products are then taken up and used by cells. HDLBP is the molecule in capillaries that facilitates the capture of chylomicrons and facilitates the interaction with LpL. It has been shown that fats in the bloodstream are not readily metabolized in the absence of HDLBP.

Alternate Names

HBPFLJ16432, HDL-binding protein, high density lipoprotein binding protein, High density lipoprotein-binding protein, PRO2900, VGLvigilin

Gene Symbol

HDLBP

Additional HDLBP Products

Product Documents for HDLBP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDLBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HDLBP Antibody - BSA Free

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  • Q: For NBP1-45962, do you have any paper or data of evidence for size of mature form is 19-36kDa? And why there was difference for size between NBP1-45962 (80-110kDa) and NBP1-83287 (140kDa)?

    A: Our HDLBP antibody #NBP1-45962 was generated as a collaborative project, so that I do not have direct access to its QC data. I have already passed your request about the research paper/reference to our collaborator who is the originator of this antibody. Moreover, I am looking into the details of why this product was discontinued, and I will let you know as soon as I have any further information. The canonical isoform of HDLBP protein is 1,268 amino acids long and it is processed further whereby the initiator methionine is removed. Its expected molecular weight is 141kD, however, the observed molecular weight may vary because of the posttranslational modifications involved (Acetylation and Phosphoprotein in case of HDLBP). Moreover, it is not very uncommon to see 10-20% change in the expected VS observed molecular weight of proteins in WB assay, especially for the high molecular weight proteins!

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