HDLBP Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05989

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDLBP Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human HDLBP fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HDLBP Antibody - BSA Free

Western Blot: HDLBP Antibody [NBP3-05989]

Western Blot: HDLBP Antibody [NBP3-05989]

Western Blot: HDLBP Antibody [NBP3-05989] - Anti-HDLBP rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:141 kDaObserved band size:160 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989] - Immunochemical staining of human HDLBP in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989]

Immunohistochemistry-Paraffin: HDLBP Antibody [NBP3-05989] - Immunochemical staining of human HDLBP in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: HDLBP Antibody [NBP3-05989]

Immunoprecipitation: HDLBP Antibody [NBP3-05989]

Immunoprecipitation: HDLBP Antibody [NBP3-05989] - HDLBP was immunoprecipitated using:Lane A: 0.5 mg HeLa Whole Cell Lysate4 uL anti-HDLBP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-HDLBP rabbit polyclonal antibody, at 1:100 dilutionSecondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 141 kDaObserved band size: 160 kDa

Applications for HDLBP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDLBP

HDL-binding protein 1 (HDLBP) is a novel protein that may be involved in the regulation of the delivery of fats to cells for energy and storage. Digested fats travel to the small intestine, where they are packaged into chylomicrons (particles filled with triglycerides). Chylomicrons then travel through the bloodstream and deliver triglycerides to tissues that are hungry for fuel or to adipose tissue for energy storage. Triglycerides are broken down or hydrolyzed by the enzyme lipoprotein lipase (LpL). The triglyceride breakdown products are then taken up and used by cells. HDLBP is the molecule in capillaries that facilitates the capture of chylomicrons and facilitates the interaction with LpL. It has been shown that fats in the bloodstream are not readily metabolized in the absence of HDLBP.

Alternate Names

HBPFLJ16432, HDL-binding protein, high density lipoprotein binding protein, High density lipoprotein-binding protein, PRO2900, VGLvigilin

Gene Symbol

HDLBP

Additional HDLBP Products

Product Documents for HDLBP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HDLBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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