HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44175

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12917

Format

Azide and BSA Free
View all HE4/WFDC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q14508, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Analysis in human salivary gland and tonsil tissues using NBP3-44175 antibody. Corresponding HE4/WFDC2 RNA-seq data are presented for the same tissues.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human kidney shows strong cytoplasmic positivity in a subset cells in tubules.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human endometrial cancer shows strong cytoplasmic positivity in tumor cells.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human salivary gland shows strong cytoplasmic positivity in interlobular ducts.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human ovarian cancer (papillary carcinoma) shows moderate to strong cytoplasmic positivity in tumor cells.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human ovarian cancer (endometrioid carcinoma) shows moderate cytoplasmic positivity in tumor cells.
HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP3-44175]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HE4/WFDC2 Antibody (CL12917) - Azide and BSA Free

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  • Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

    A:

    At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

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