HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71599

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E12

Format

Azide and BSA Free
View all HE4/WFDC2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 31-124 of human WFDC2 (NP_006094) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

Western Blot: HE4/WFDC2 Antibody (OTI1E12)Azide and BSA Free [NBP2-71599]

Western Blot: HE4/WFDC2 Antibody (OTI1E12)Azide and BSA Free [NBP2-71599]

Western Blot: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WFDC2.
Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599]

Immunohistochemistry: HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free [NBP2-71599] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HE4/WFDC2 Antibody (OTI1E12) - Azide and BSA Free

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  • Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

    A:

    At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

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