Hemoglobin beta Antibody (7B12) - Azide and BSA Free

Novus Biologicals | Catalog # H00003043-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7B12

Format

Azide and BSA Free
View all Hemoglobin beta Primary Antibodies »

Product Specifications

Immunogen

HBB (AAH07075, 38 a.a. ~ 147 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALPHKYH

Specificity

HBB - hemoglobin, beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02]

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02]

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02] - Analysis of monoclonal antibody to HBB on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02]

ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02]

ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02] - Detection limit for recombinant GST tagged HBB is approximately 0.1ng/ml as a capture antibody.

Applications for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hemoglobin beta

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Alternate Names

beta globin chain, beta-globin, CD113t-C, HBBB, HBD, Hemoglobin beta chain, hemoglobin subunit beta, hemoglobin, beta

Entrez Gene IDs

3043 (Human)

Gene Symbol

HBB

OMIM

141900 (Human)

UniProt

AAH07075 (Human)

Additional Hemoglobin beta Products

Product Documents for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Hemoglobin beta Antibody (7B12) - Azide and BSA Free

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  • Q: What are the concentrations of H00003043M02, H00003043M12 and NBP2-32769. Also, do these antibodies  detect glycosylated hemoglobin specifically.

    A: For both H00003043M02 and H00003043M12 have the concentration of 1.0 mg/ml. H00003043M02 and H00003043M12 have not been verified for the purpose of detecting glycosylated hemoglobin.
    If you look for antibodies specifically for detection of glycosylated hemoglobin, I’ll recommend the followings:
    MAB0030-M06 Human HbA1c monoclonal antibody, clone 1D3
    MAB0030-M06A Human HbA1c monoclonal antibody, clone 1D3
    MAB0030-M01 Human HbA1c monoclonal antibody, clone 1E4
    MAB0030-M03 Human HbA1c monoclonal antibody, clone 2A10
    MAB0030-M05 Human HbA1c monoclonal antibody, clone 2A3
    MAB0030-M02 Human HbA1c monoclonal antibody, clone 2H5
    MAB0030-M04 Human HbA1c monoclonal antibody, clone 4B10
    MAB0030-M07 Human HbA1c monoclonal antibody, clone 7E2
    For NBP2-32769, the concentration is 6.3 mg/ml for the current lot and it reacts with glycated hemoglobin and it does not cross-react with non-glycated hemoglobin.

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