Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92010

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:QRYQYKRQLERREQRLRSREERLLHRAKEALPREDADEPGRVPTEDYMSHIIEKW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody - BSA Free

Western Blot: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody [NBP1-92010]

Western Blot: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody [NBP1-92010]

Western Blot: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody [NBP1-92010] - Analysis in control (vector only transfected HEK293T lysate) and HS6ST1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody [NBP1-92010]

Immunocytochemistry/ Immunofluorescence: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody [NBP1-92010]

Immunocytochemistry/Immunofluorescence: HS6ST1 Antibody [NBP1-92010] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.

Applications for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

HS6ST1 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. It is expressed in foetal brain and liver and is thought to play a significant role in axonal guidance through sulphation of heparan sulphate proteoglycans, with HS6ST1 expression mirroring that of Slit1 and Slit2.

Alternate Names

DKFZp547H098, EC 2.8.2, EC 2.8.2.-, FLJ25392, heparan sulfate 6-O-sulfotransferase 1, heparan-sulfate 6-sulfotransferase, HS6ST-1, HS6STheparan-sulfate 6-O-sulfotransferase 1, MGC116899, MGC116901

Gene Symbol

HS6ST1

Additional Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Products

Product Documents for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail