Hepatocyte Specific Antigen Antibody (HepPar1)

Novus Biologicals | Catalog # NBP2-45272

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HepPar1
View all Hepatocyte Specific Antigen Primary Antibodies »

Product Specifications

Immunogen

Extract of a formalin-fixed, rejected-allograft of a human liver

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27542264).

Localization

Finely granular cytoplasmic

Marker

Hepatocellular Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08970)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Hepatocyte Specific Antigen Antibody (HepPar1)

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Formalin-fixed, paraffin-embedded human Liver stained with Hepatocyte Specific Antigen Antibody (HepPar1).
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Human Hepatocellular Carcinoma stained with HepPar-1 Monoclonal Antibody (HepPar1).

Applications for Hepatocyte Specific Antigen Antibody (HepPar1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Western Blot reported in scientific literature (PMID:31998631).

Reviewed Applications

Read 1 review rated 5 using NBP2-45272 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Hepatocyte Specific Antigen

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Alternate Names

Hepatocyte, HSA

Additional Hepatocyte Specific Antigen Products

Product Documents for Hepatocyte Specific Antigen Antibody (HepPar1)

Certificate of Analysis

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Product Specific Notices for Hepatocyte Specific Antigen Antibody (HepPar1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hepatocyte Specific Antigen Antibody (HepPar1)

Customer Reviews for Hepatocyte Specific Antigen Antibody (HepPar1) (1)

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Customer Images

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  • Hepatocyte Specific Antigen Antibody (HepPar1)
    Name: Junhua Yang
    Application: Western Blot
    Sample Tested: Liver tissue and human liver cell
    Species: Mouse, and human and Human
    Verified Customer | Posted 12/01/2016
    HepPar1 Ab: Novus# NBP2-45272, 1:500 3%milk. Goat anti mouse 1:4000, size 165kd Sample: Human liver tissue
    Hepatocyte Specific Antigen Antibody (HepPar1) NBP2-45272

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Protocols

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