Hexokinase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87480

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hexokinase 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 1 Antibody - BSA Free

Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480] - Analysis using Anti-HK1 antibody NBP1-87480 (A) shows similar pattern to independent antibody NBP1-85789 (B).
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-87480]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-87480]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-87480] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480] - Analysis in human cell line PC-3.
Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480]

Western Blot: Hexokinase 1 Antibody [NBP1-87480] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Hexokinase 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexokinase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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