Hexokinase 2 Antibody (OTI4C5)

Novus Biologicals | Catalog # NBP2-02272

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C5
View all Hexokinase 2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32393782). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Human Hexokinase-2 protein is 74% identical with Hexokinase-1 and our Hexokinase 2 antibody (clone 4C5) is not expected to crossreact with Hexokinase-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 2 Antibody (OTI4C5)

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0021.jpg
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.
Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.
Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.
Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272]

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.

Applications for Hexokinase 2 Antibody (OTI4C5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Alternate Names

HK2, HKII, HXK2

Entrez Gene IDs

1146 (Human)

Gene Symbol

CHRNG

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (OTI4C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Hexokinase 2 Antibody (OTI4C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hexokinase 2 Antibody (OTI4C5)

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Protocols

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