Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70891

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C5

Format

Azide and BSA Free
View all Hexokinase 2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free

Western Blot: Hexokinase 2 Antibody (OTI4C5)Azide and BSA Free [NBP2-70891]

Western Blot: Hexokinase 2 Antibody (OTI4C5)Azide and BSA Free [NBP2-70891]

Western Blot: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinas
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.
Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.
Western Blot: Hexokinase 2 Antibody (OTI4C5)Azide and BSA Free [NBP2-70891]

Western Blot: Hexokinase 2 Antibody (OTI4C5)Azide and BSA Free [NBP2-70891]

Western Blot: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.
Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891]

Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free [NBP2-70891] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.

Applications for Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Hexokinase 2 Antibody (OTI4C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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