Hexokinase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16814

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Porcine (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hexokinase 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hexokinase 2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (86%), Chicken (88%).

Localization

Mitochondrion outer membrane (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814]

Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814]

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814]

Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Hexokinase 2 Antibody - BSA Free

Western Blot: Hexokinase 2 Antibody [NBP2-16814] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (NBP2-16814) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hexokinase 2 Antibody - BSA Free

Western Blot: Hexokinase 2 Antibody [NBP2-16814] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (NBP2-16814) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hexokinase 2 Antibody - BSA Free

Western Blot: Hexokinase 2 Antibody [NBP2-16814] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (NBP2-16814) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Hexokinase 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

UniProt

P52789

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexokinase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hexokinase 2 Antibody - BSA Free

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Protocols

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