HIBCH Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15496

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HIBCH Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-330 of human HIBCH (NP_055177.2). DAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIBCH Antibody - Azide and BSA Free

HIBCH Antibody - Azide and BSA Free

Western Blot: HIBCH Antibody - Azide and BSA Free [HIBCH] -

Western Blot: HIBCH Antibody - Azide and BSA Free [HIBCH] - Western blot analysis of various lysates, using HIBCH Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: HIBCH Antibody - Azide and BSA Free [NBP3-15496]

Immunocytochemistry/ Immunofluorescence: HIBCH Antibody - Azide and BSA Free [NBP3-15496]

Immunocytochemistry/Immunofluorescence: HIBCH Antibody [NBP3-15496] - Immunofluorescence analysis of U-2 OS cells using HIBCH antibody (NBP3-15496) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HIBCH Antibody - Azide and BSA Free [NBP3-15496]

Immunocytochemistry/ Immunofluorescence: HIBCH Antibody - Azide and BSA Free [NBP3-15496]

Immunocytochemistry/Immunofluorescence: HIBCH Antibody [NBP3-15496] - Immunofluorescence analysis of L929 cells using HIBCH antibody (NBP3-15496) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for HIBCH Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIBCH

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.(supplied by OMIM)

Alternate Names

3-hydroxyisobutyryl-CoA hydrolase, EC 3.1.2.4,3-hydroxyisobutyryl-coenzyme A hydrolase3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIB-CoA hydrolase, HIBYLCOAH, HIBYL-CoA-H

Gene Symbol

HIBCH

Additional HIBCH Products

Product Documents for HIBCH Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HIBCH Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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