HIC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55846

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HIC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLD

Reactivity Notes

Mouse 80%, Rat 81%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HIC2 Antibody [NBP2-55846]

Immunocytochemistry/ Immunofluorescence: HIC2 Antibody [NBP2-55846]

Immunocytochemistry/Immunofluorescence: HIC2 Antibody [NBP2-55846] - Staining of human cell line HEK 293 shows localization to nucleus.
HIC2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: HIC2 Antibody - BSA Free [NBP2-55846]

Chromatin Immunoprecipitation-exo-Seq: HIC2 Antibody - BSA Free [NBP2-55846]

ChIP-Exo-Seq composite graph for Anti-HIC2 (NBP2-55846) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for HIC2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIC2

Transcriptional repressor

Alternate Names

HIC1-related gene on chromosome 22 protein, Hic-2, Hic-3, HRG22KIAA1020hic-2, hypermethylated in cancer 2, hypermethylated in cancer 2 protein, ZBTB30hic-3, Zinc finger and BTB domain-containing protein 30, ZNF907

Gene Symbol

HIC2

Additional HIC2 Products

Product Documents for HIC2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HIC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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