High Throughput Glutathione Assay Kit
High Throughput Glutathione Assay Kit Summary
Quantifies the amount of glutathione in mammalian cells, tissue, blood, plasma and other bodily fluids using a 96-well plate.Key Benefits
• Suitable for mammalian cells, tissue, blood, plasma and other bodily fluids.
• Formatted for 96-well plates.
• Contains sufficient reagents to assay 384 data points or to determine GSH in:
Why Use the HT Glutathione Assay Kit?
Glutathione Assay utilizes a carefully optimized enzymatic recycling method, which employs Glutathione Reductase, for the quantification of glutathione. The sulfhydryl group of glutathione reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow-colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by Glutathione Reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to the concentration of GSH in the sample. Thus, measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of the amount of glutathione in the sample.
Other Resources
7511-100-k_calculation_worksheet
Kit Contents
• Glutathione Reductase
• 25X Assay Buffer
• Reaction Mix
• 96-Well Plates
• 4 µM GSSG
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for High Throughput Glutathione Assay Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Maternal N-Acetyl-Cysteine Prevents Neonatal Hypoxia-Induced Brain Injury in a Rat Model
Authors: O Gutziet, R Iluz, H Ben Asher, L Segal, D Ben Zvi, Y Ginsberg, N Khatib, O Zmora, MG Ross, Z Weiner, R Beloosesky
International Journal of Molecular Sciences, 2021-12-20;22(24):. 2021-12-20
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Enzyme-mediated depletion of serum l-Met abrogates prostate cancer growth via multiple mechanisms without evidence of systemic toxicity
Authors: WC Lu, A Saha, W Yan, K Garrison, C Lamb, R Pandey, S Irani, A Lodi, X Lu, S Tiziani, YJ Zhang, G Georgiou, J DiGiovanni, E Stone
Proc. Natl. Acad. Sci. U.S.A., 2020-05-20;0(0):. 2020-05-20
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Egg white consumption increases GSH and lowers oxidative damage in 110-week-old geriatric mice hearts
Authors: Y Jiayu, A Botta, S Simtchouk, J Winkler, LM Renaud, H Dadlani, B Rasmussen, R Elango, S Ghosh
J. Nutr. Biochem., 2019-10-26;76(0):108252. 2019-10-26
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Tissue-specific responses of antioxidant pathways to poor hygiene conditions in growing pigs divergently selected for feed efficiency
Authors: K Sier?ant, MH Perruchot, E Merlot, N Le Floc'h, F Gondret
BMC Vet. Res., 2019-10-16;15(1):341. 2019-10-16
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Sophocarpine Attenuates LPS-Induced Liver Injury and Improves Survival of Mice through Suppressing Oxidative Stress, Inflammation, and Apoptosis
Authors: Z Jiang, Y Meng, L Bo, C Wang, J Bian, X Deng
Mediators Inflamm., 2018-05-16;2018(0):5871431. 2018-05-16
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Increased VEGF-A promotes multiple distinct aging diseases of the eye through shared pathomechanisms.
Authors: Marneros A
EMBO Mol Med, 2016-03-01;8(3):208-31. 2016-03-01
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Characterization of a c-Rel Inhibitor That Mediates Anticancer Properties in Hematologic Malignancies by Blocking NF-kappaB-Controlled Oxidative Stress Responses.
Authors: Shono Y, Tuckett A, Liou H, Doubrovina E, Derenzini E, Ouk S, Tsai J, Smith O, Levy E, Kreines F, Ziegler C, Scallion M, Doubrovin M, Heller G, Younes A, O'Reilly R, van den Brink M, Zakrzewski J
Cancer Res, 2016-01-07;76(2):377-89. 2016-01-07
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Rosiglitazone causes cardiotoxicity via peroxisome proliferator-activated receptor gamma-independent mitochondrial oxidative stress in mouse hearts.
Authors: He H, Tao H, Xiong H, Duan S, McGowan F, Mortensen R, Balschi J
Toxicol Sci, 2014-01-21;138(2):468-81. 2014-01-21
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Oxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells.
Authors: Brem R, Karran P
Cancer Res, 2012-07-20;72(18):4787-95. 2012-07-20
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Association between exhaled breath condensate nitrate + nitrite levels with ambient coarse particle exposure in subjects with airways disease.
Authors: Manney S, Meddings C, Harrison R, Mansur A, Karakatsani A, Analitis A, Katsouyanni K, Perifanou D, Kavouras I, Kotronarou N, de Hartog J, Pekkanen J, Hameri K, ten Brink H, Hoek G, Ayres J
Occup Environ Med, 2012-07-05;69(9):663-9. 2012-07-05
FAQs
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Does the HT Glutathione Assay kit distinguish between oxidized and reduced glutathione?
Yes, the HT Glutathione Assay kit does measure total glutathione as well as oxidized and reduced glutathione.
The addition of 4-vinylpyridine to the standard or cell extract blocks reduced glutathione (GSH) but not oxidized glutathione (GSSG). Thus the only form remaining after 4-vinylpyridine treatment is GSSG. The reduced form (GSH) is calculated by first determining total glutathione with the kit, and then treating with 4-vinylpyridine. Subtracting GSSG from total glutathione then gives the reduced GSH concentration.
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