High Throughput Glutathione Reductase Assay
High Throughput Glutathione Reductase Assay Summary
Key Benefits
• Suitable for cell lysates, erythrocyte lysates, and tissue homogenate
• Sufficient reagents for 500 assays
• 96 well format for high throughput screening
Why Use the Glutathione Reductase Assay Kit?
The Glutathione Reductase Assay Kit is a spectrophotometric assay in which the oxidation of NADPH to NADP+ is monitored by the decrease in absorbance at 340 nm. This rate of decrease in absorbance at 340 nm is directly proportional to the glutathione reductase activity in the sample because the enzyme is present at rate limiting concentrations. This kit provides the user with all the reagents and plates to easily and rapidly assay for glutathione reductase in cell and tissue extracts.
Other Resources
7513-500-k_calculation_worksheet
Kit Contents
• NADPH
• Glutathione Reductase (1U/mL)
• 20% Triton X-100
• 96-Well Plate
• 10X GR Buffer
• GSSG Solution
Specifications
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
Citations for High Throughput Glutathione Reductase Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Altered glutathione homeostasis in heart augments cardiac lipotoxicity associated with diet-induced obesity in mice.
Authors: Ghosh S, Sulistyoningrum D, Glier M, Verchere C, Devlin A
J Biol Chem, 0;286(49):42483-93. 0
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Amount and source of dietary copper affects small intestine morphology, duodenal lipid peroxidation, hepatic oxidative stress,and mRNA expression of hepatic copper regulatory proteins in weanling pigs.
Authors: Fry R, Ashwell M, Lloyd K, O'Nan A, Flowers W, Stewart K, Spears J
J Anim Sci, 0;90(9):3112-9. 0
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Mitochondrial oxidative stress-induced epigenetic modifications in pancreatic epithelial cells.
Authors: Mishra P, Raghuram G, Jain D, Jain S, Khare N, Pathak N
Int J Toxicol, 0;33(2):116-29. 0
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