Glutathione Reductase (GR) is a homodimeric flavoprotein disulfide oxidoreductase that plays a critical role in the prevention of oxidative damage within the cell. Glutathione Reductase helps to maintain appropriate levels of intracellular reduced glutathione (GSH) which is essential for maintaining the normal structure of red blood cells and for keeping hemoglobin in the ferrous state. Glutathione Reductase together with its co-factor, NADPH, catalyzes the reduction of oxidized glutathione (glutathione disulfide, GSSG) to glutathione. GSH is also a reactant for Glutathione Peroxidase, which converts hydrogen peroxide hydrogen peroxide (H2O2) into water. Glutathione Reductase is expressed as both mitochondrial and cytosolic isoforms, as determined by the presence or absence of an N-terminal transit peptide.
High Throughput Glutathione Reductase Assay
R&D Systems | Catalog # 7513-500-K
Quantification of glutathione reductase activity.
Discontinued Product
7513-500-K has been discontinued.
View all Glutathione Reductase products.
Key Product Details
Features
Designed to measure cellular levels of glutathione reductase using a 96-well plate.
Key Benefits
- Suitable for cell lysates, erythrocyte lysates, and tissue homogenate
- Sufficient reagents for 500 assays
- 96 well format for high throughput screening
Species
Multi-Species
Product Summary for High Throughput Glutathione Reductase Assay
Why Use the Glutathione Reductase Assay Kit?
The Glutathione Reductase Assay Kit is a spectrophotometric assay in which the oxidation of NADPH to NADP+ is monitored by the decrease in absorbance at 340 nm. This rate of decrease in absorbance at 340 nm is directly proportional to the glutathione reductase activity in the sample because the enzyme is present at rate limiting concentrations. This kit provides the user with all the reagents and plates to easily and rapidly assay for glutathione reductase in cell and tissue extracts.
Other Resources
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Kit Contents for High Throughput Glutathione Reductase Assay
- NADPH
- Glutathione Reductase (1U/mL)
- 20% Triton X-100
- 96-Well Plate
- 10X GR Buffer
- GSSG Solution
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Glutathione Reductase
Alternate Names
GLUR, GRase, GRD1, GSR, HEL-752
Additional Glutathione Reductase Products
Product Documents for High Throughput Glutathione Reductase Assay
Related Research Areas
Citations for High Throughput Glutathione Reductase Assay
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FAQs for High Throughput Glutathione Reductase Assay
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A: The assay protocol is validated for isolating Glutathione Peroxidase from fresh tissue. We have not tested frozen tissue in this assay. Fresh tissue is recommended because single cell suspension from tissue extracts is needed to eventually prepare cytosolic extracts. It is very difficult to make single cell extracts from frozen tissue and most of the cells have likely already lysed from freezing. We recommend isolating the cytosolic extracts prior to freezing the samples.
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A: This kit may be used to measure Glutathione Reductase activity in serum or plasma samples within the detection range of the assay.
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A: The source of standard in the kit is S. cerevisiae.
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A: Over 95% of the hemoglobin can be removed by adding Zinc Sulfate to a final concentration of 500 uM and centrifuging to remove the precipitate. For more information, please see: Sajan MP, and Kulkarni AP. "A simple and rapid method for hemoglobin removal from mammalian tissue cytosol by zinc sulfate and its application to the study of Glutathione Treansferase". Toxicology Methods, 7:1051 (1997).
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A: A 96-well, flat bottom ELISA plate can be substituted for the plates supplied.