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High Throughput Glutathione Reductase Assay

7513-500-K 3 Citations Datasheet / COA / SDS
Quantification of glutathione reductase activity
Catalog # Availability Size / Price Qty
7513-500-K
Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Background

High Throughput Glutathione Reductase Assay Summary

Designed to measure cellular levels of glutathione reductase using a 96-well plate.

Key Benefits

• Suitable for cell lysates, erythrocyte lysates, and tissue homogenate
• Sufficient reagents for 500 assays
• 96 well format for high throughput screening

Why Use the Glutathione Reductase Assay Kit?

The Glutathione Reductase Assay Kit is a spectrophotometric assay in which the oxidation of NADPH to NADP+ is monitored by the decrease in absorbance at 340 nm. This rate of decrease in absorbance at 340 nm is directly proportional to the glutathione reductase activity in the sample because the enzyme is present at rate limiting concentrations. This kit provides the user with all the reagents and plates to easily and rapidly assay for glutathione reductase in cell and tissue extracts.

Other Resources

7513-500-k_calculation_worksheet

Kit Contents

• NADPH
• Glutathione Reductase (1U/mL)
• 20% Triton X-100
• 96-Well Plate
• 10X GR Buffer
• GSSG Solution

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for High Throughput Glutathione Reductase Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Altered glutathione homeostasis in heart augments cardiac lipotoxicity associated with diet-induced obesity in mice.
    Authors: Ghosh S, Sulistyoningrum D, Glier M, Verchere C, Devlin A
    J Biol Chem, 0;286(49):42483-93.  0
  2. Amount and source of dietary copper affects small intestine morphology, duodenal lipid peroxidation, hepatic oxidative stress,and mRNA expression of hepatic copper regulatory proteins in weanling pigs.
    Authors: Fry R, Ashwell M, Lloyd K, O'Nan A, Flowers W, Stewart K, Spears J
    J Anim Sci, 0;90(9):3112-9.  0
  3. Mitochondrial oxidative stress-induced epigenetic modifications in pancreatic epithelial cells.
    Authors: Mishra P, Raghuram G, Jain D, Jain S, Khare N, Pathak N
    Int J Toxicol, 0;33(2):116-29.  0

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