High Throughput Superoxide Dismutase Assay Kit

Determines relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan
Catalog # Availability Size / Price Qty
7501-500-K
Product Details
Citations (2)
FAQs
Reviews

High Throughput Superoxide Dismutase Assay Kit Summary

Analyzes Superoxide Dismutase (SOD) activity in cell extracts using a 96-well plate.

Why Use the HT Superoxide Dismutase Assay Kit?

In the Superoxide Dismutase Assay, ions generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in experimental samples. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample.

Other Resources

7501-500-k_calculation_worksheet

Kit Contents

• SOD Standard
• Xanthine Oxidase (XOD)
• 10X SOD Buffer
• 10X Xanthine Solution
• 20% Triton X-100
• WST-1 Reagent
• 96-Well Plate

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for High Throughput Superoxide Dismutase Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Authors: X Li, Y Chen, J Zhao, J Shi, M Wang, S Qiu, Y Hu, Y Xu, Y Cui, C Liu, C Liu
    Oxid Med Cell Longev, 2019;2019(0):9706792.  2019
  2. Actions of Inonotus obliquus against Hyperuricemia through XOD and Bioactives Screened by Molecular Modeling
    Authors: T Yong, S Chen, D Liang, D Zuo, X Diao, C Deng, Y Wu, H Hu, Y Xie, D Chen
    Int J Mol Sci, 2018;19(10):.  2018

FAQs

  1. Can this kit be used to measure serum or plasma samples?

    • This kit may be used to measure SOD activity in serum or plasma samples within the detection range of the assay.

  2. Can this kit be used to measure saliva samples?

    • This kit may be used to measure SOD activity in saliva samples within the detection range of the assay.

  3. What is the Lower Limit of Detection (LLD) of this kit?

    • An exact LLD has not been established for this kit. However, the lowest amount that is recommended for the standard curve is 0.1 Unit of SOD. The standard curve is based on a purified enzyme.

  4. Can frozen tissues be used in this assay?

    • We have not tested frozen tissue in this assay. The protocol is based on isolating SOD from fresh tissue. We recommend isolating the cytosolic extracts prior to freezing the samples. Fresh tissue is recommended because single cell suspension from  tissue extracts is needed to eventually prepare cytosolic extracts. It is very difficult to make single cell extracts from frozen tissue and most of the cells have likely already lysed from freezing. 

  5. Which form of SOD does this kit detect?

    • This kit detects all SOD forms.

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