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High Throughput Superoxide Dismutase Assay Kit

7501-500-K 2 Citations Datasheet / COA / SDS
Determines relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan
Catalog # Availability Size / Price Qty
7501-500-K
Product Details
Citations (2)
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Summary Product Datasheets Background

High Throughput Superoxide Dismutase Assay Kit Summary

Analyzes Superoxide Dismutase (SOD) activity in cell extracts using a 96-well plate.

Why Use the HT Superoxide Dismutase Assay Kit?

In the Superoxide Dismutase Assay, ions generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in experimental samples. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample.

Other Resources

7501-500-k_calculation_worksheet

Kit Contents

• SOD Standard
• Xanthine Oxidase (XOD)
• 10X SOD Buffer
• 10X Xanthine Solution
• 20% Triton X-100
• WST-1 Reagent
• 96-Well Plate

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for High Throughput Superoxide Dismutase Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Authors: X Li, Y Chen, J Zhao, J Shi, M Wang, S Qiu, Y Hu, Y Xu, Y Cui, C Liu, C Liu
    Oxid Med Cell Longev, 2019;2019(0):9706792.  2019
  2. Actions of Inonotus obliquus against Hyperuricemia through XOD and Bioactives Screened by Molecular Modeling
    Authors: T Yong, S Chen, D Liang, D Zuo, X Diao, C Deng, Y Wu, H Hu, Y Xie, D Chen
    Int J Mol Sci, 2018;19(10):.  2018

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