HIPPI Antibody

Name: HIPPI Antibody
Brand: Novus Biologicals
SKU: NBP1-28913
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-28913

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-EQPQEYDDPNATISN corresponding to internal region according to NP_060480.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HIPPI Antibody

Western Blot: HIPPI Antibody [NBP1-28913]

Western Blot: HIPPI Antibody [NBP1-28913] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: HIPPI Antibody [NBP1-28913]

Immunohistochemistry-Paraffin: HIPPI Antibody [NBP1-28913] - Staining of paraffin embedded Human Testes tissue with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for HIPPI Antibody

Application

Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/mL

Application Notes

WB: Approx. 49 kDa band observed in human and mouse brain lysates (calculated MW of 49.1 kDa band according to human NP_060480.1 and of 48.8 kDa band according to mouse NP_082956.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIPPI

HIPPI is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and tri

Alternate Names

Dermal papilla-derived protein 8, DERP8, ESRRBL1huntingtin interacting protein-1 interacting protein, estrogen-related receptor beta like 1, Estrogen-related receptor beta-like protein 1, FLJ10147, HIPPIHIP1-interacting protein, intraflagellar transport 57 homolog (Chlamydomonas), intraflagellar transport protein 57 homolog, MHS4R2HIP1 protein interactor

Entrez Gene IDs

55081 (Human); 73916 (Mouse); 303968 (Rat)

Gene Symbol

IFT57

UniProt

NP_060480.1 (Human)

Additional HIPPI Products

Product Documents for HIPPI Antibody

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Certificate of Analysis

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Product Specific Notices for HIPPI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HIPPI Antibody

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