HISPPD2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89693

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HISPPD2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CLENSEEVSQPCQGVSVEVGKLVHKFHVGVGSLVQETLVEVGSPAEEIPEEVIQPYQEFSVEVGRLAQETSAINLLSQGIPEIDKPS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29590114).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HISPPD2A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - Validation of anti-PPIP5K2 and anti-PPIP5K1 antibodies specific in vitro. (A) Anti-PPIP5K2 antibody immunofluorescence signal coincides with the signal produced by GFP-tagged WT PPIP5K2, and p.Arg837His variant harboring PPIP5K2 expressed in COS7 cells. (B) The anti-PPIP5K1 antibody immunofluorescence signal coincides with the GFP-tagged PPIP5K1 signal, but not with fluorescently tagged PPIP5K2 expressed in COS7 cells. (C) Western blot tested indicated the specific bands of sizes that correspond to full-length PPIP5K2 around 140 kDa. (D) Western blot on whole protein lysates from mice tissues with specific variants. Citation: Yousaf R, Gu C, Ahmed ZM, Khan SN, Friedman TB, Riazuddin S, et al. (2018) Mutations in Diphosphoinositol-Pentakisphosphate Kinase PPIP5K2 are associated with hearing loss in human and mouse. PLoS Genet 14(3): e1007297
Immunohistochemistry-Paraffin: HISPPD2A Antibody [NBP1-89693]

Immunohistochemistry-Paraffin: HISPPD2A Antibody [NBP1-89693]

Immunohistochemistry-Paraffin: HISPPD2A Antibody [NBP1-89693] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/ Immunofluorescence: HISPPD2A Antibody [NBP1-89693]

Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - HISPPD2A/PPIP5K1 antibody was used at 1:200 dilution for localizing PPIP5K2 protein in sensory and non-sensory cells of mouse inner ear using confocal microscopy analysis. Figure A shows the real-time PCR expression of Ppip5k2 in adult (P150) mice, normalized against Gapdh (Delta CT) and Ppip5k1 expression (Delta CT). In Figure B, a cross-section through one of the coils of inner ear shows diffuse cytoplasmic immunolabeling of PPIP5K2 throughout the cochlear duct, including spiral ganglion neurons (SG), organ of Corti (OC), and stria vascularis (SV). Figure C shows the expression of PPIP5K2 at the ages tested in WT mice, from early postnatal day P16, up to 9 months of age. Scale bars: 100um (panel A), and 20um (panel C).

Applications for HISPPD2A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HISPPD2A

Inositol phosphates (IPs) and diphosphoinositol phosphates (PP-IPs), also known as inositol pyrophosphates, act as cell signaling molecules. HISPPD2A has both IP6 kinase (EC 2.7.4.21) and PP-IP5 (also called IP7) kinase (EC 2.7.4.24) activities that produce the high-energy pyrophosphates PP-IP5 and PP2-IP4 (also called IP8), respectively (Fridy et al., 2007 [PubMed 17690096]).[supplied by OMIM]

Alternate Names

diphosphoinositol pentakisphosphate kinase 1, histidine acid phosphatase domain-containing protein 2A, inositol pyrophosphate synthase 1, VIP1

Gene Symbol

PPIP5K1

Additional HISPPD2A Products

Product Documents for HISPPD2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HISPPD2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HISPPD2A Antibody - BSA Free

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Protocols

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