HIST1H2BC Antibody (CL5101) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44254

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5101

Format

Azide and BSA Free
View all HIST1H2BC Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P62807, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HIST1H2BC Antibody (CL5101) - Azide and BSA Free

HIST1H2BC Antibody (CL5101) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in lamina propria cells.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular and stromal cells.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Staining of human prostate shows moderate to strong nuclear positivity in glandular and smooth muscle cells.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Western Blot: HIST1H2BC Antibody [NBP3-44254]

Western Blot: HIST1H2BC Antibody [NBP3-44254]

Analysis in human cell line HEL.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Immunohistochemistry-Paraffin: HIST1H2BC Antibody [NBP3-44254]

Staining of human striated muscle shows strong nuclear positivity in striated muscle fibers.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Western Blot: HIST1H2BC Antibody [NBP3-44254]

Western Blot: HIST1H2BC Antibody [NBP3-44254]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
HIST1H2BC Antibody (CL5101) - Azide and BSA Free Western Blot: HIST1H2BC Antibody [NBP3-44254]

Western Blot: HIST1H2BC Antibody [NBP3-44254]

Analysis of purified human recombinant histone H2B, H2A, H3 and H4.

Applications for HIST1H2BC Antibody (CL5101) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIST1H2BC

HIST1H2BC is 126 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, hemochromatosis, and immunodeficiency. HIST1H2BC has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the histone modification, signal transduction of Activin A, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Alternate Names

H2B histone family, member K, H2B/a, H2B/g, H2B/kH2B/h, H2BFA, H2BFG, H2BFH, H2BFKH2B/l, H2BFL, HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, histone 1, H2bi, histone cluster 1, H2bi, histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l

Gene Symbol

HIST1H2BC

Additional HIST1H2BC Products

Product Documents for HIST1H2BC Antibody (CL5101) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HIST1H2BC Antibody (CL5101) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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