Histamine H1R Antibody

Novus Biologicals | Catalog # NB600-795

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Histamine H1 R Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CNENFKKTFKRILH corresponding to C-Terminus according to NP_001091681.1, NP_001091682.1, NP_001091683.1.

Specificity

Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Histamine H1R Antibody

Flow Cytometry: Histamine H1R Antibody [NB600-795]

Flow Cytometry: Histamine H1R Antibody [NB600-795]

Flow Cytometry: Histamine H1R Antibody [NB600-795] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Histamine H1R Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:32000
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID: 32152328).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histamine H1 R

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Itsvarious actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless untilrecently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupledreceptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due tocontraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in thecentral nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified.(provided by RefSeq)

Long Name

Histamine H1 Receptor

Alternate Names

H1-R, Histamine H1R, HRH1

Entrez Gene IDs

3269 (Human); 15465 (Mouse); 24448 (Rat)

Gene Symbol

HRH1

UniProt

NP_000852.1 (Human)

Additional Histamine H1 R Products

Product Documents for Histamine H1R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Histamine H1R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histamine H1R Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Histamine H1R Antibody

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  • Q: I would like to know if HRH1 antibody (NB600-795) and HRH3 antibody (NBP1-71095) have cross-reactivity with mouse or whether the reactivity is only predicted? I'd like to test for presence of these receptors in our mouse neural stem cells.

    A: For NB600-795, I am showing the reactivity of Mouse, Rat, Dog, and Cow are based upon homology, but will still be guaranteed by us since they are shown on the datasheet. Therefore if it does not work in your mouse samples for this one we would be happy to send you a replacement or refund. The second antibody NBP1-71095 was also determined to have Rat and Mouse reactivity based on sequence homology, but like the first will be guaranteed for the applications listed for those species.

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