Histamine N-Methyltransferase/HNMT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89495

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histamine N-Methyltransferase/HNMT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histamine N-Methyltransferase/HNMT Antibody - BSA Free

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP1-89495] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Histamine N-Methyltransferase/HNMT Antibody - BSA Free Western Blot: Histamine N-Methyltransferase/HNMT Antibody - BSA Free [NBP1-89495]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody - BSA Free [NBP1-89495]

Analysis in human cell line HepG2.

Applications for Histamine N-Methyltransferase/HNMT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histamine N-Methyltransferase/HNMT

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Long Name

Histamine N-Methyltransferase

Alternate Names

Histamine NMethyltransferase, HMT, HNMT, HNMT-S1, HNMT-S2

Gene Symbol

HNMT

Additional Histamine N-Methyltransferase/HNMT Products

Product Documents for Histamine N-Methyltransferase/HNMT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histamine N-Methyltransferase/HNMT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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