Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

Novus Biologicals | Catalog # NBP2-59269

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C.15200201

Format

BSA Free
View all Histone Deacetylase 2/HDAC2 Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Chromatin Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - ChIP assays were performed using human HeLa cells, the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.

Applications for Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1 ug

Chromatin Immunoprecipitation Sequencing

1ug/ChIP

Immunocytochemistry/ Immunofluorescence

1:500

Immunofluorescence

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail