Histone H2a Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56346

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Human, Mouse, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A.

Reactivity Notes

Use in Sheep reported in scientific literature (PMID:32849530).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2a Antibody - BSA Free

Western Blot: Histone H2a Antibody [NB100-56346]

Western Blot: Histone H2a Antibody [NB100-56346]

Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346]

Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346]

Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: Histone H2a Antibody [NB100-56346]

Western Blot: Histone H2a Antibody [NB100-56346]

Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.
Histone H2a Antibody - BSA Free

Western Blot: Histone H2a Antibody - BSA Free [NB100-56346] -

Histone H3 and H4 protein expression is regulated by CTSV. (A) Western blotting analysis of histone protein expression shows a reduction in histone H3 and H4 in CTSV depleted cells, with no impact on histones H1, H2a and H2b. (B) Confirmation of CTSV impact on histone H3 and H4 was ascertained using rescue experiments and the re-expression of CTSV in shCTSV-1 cells. GAPDH expression was used as an internal loading control and data presented is representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38026973), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Histone H2a Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

reported in scientific literature (PMID 32849530)

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 ug/ml
Application Notes
A 14 kDa band is observed.

Reviewed Applications

Read 1 review rated 4 using NB100-56346 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2A

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.

Long Name

H2A Histone Family

Alternate Names

H2A1B, HIST1H2AB

Entrez Gene IDs

92815 (Human)

Gene Symbol

H2AC20

UniProt

P04908 (Human)

Additional Histone H2A Products

Product Documents for Histone H2a Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Histone H2a Antibody - BSA Free

Customer Reviews for Histone H2a Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 and HCT116 cells whole cell extract
    Species: Human
    Verified Customer | Posted 07/31/2014
    WB for hsitone H2A in MCF-7 and HCT116 cells
    Histone H2a Antibody - BSA Free NB100-56346

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