Histone H2AX Antibody (3F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00003014-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F4

Format

Azide and BSA Free
View all Histone H2AX Primary Antibodies »

Product Specifications

Immunogen

H2AFX (AAH04915, 1 a.a. ~ 143 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY

Specificity

H2AFX (3F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Histone H2AX Antibody (3F4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (3F4) [H00003014-M05]

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (3F4) [H00003014-M05]

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (3F4) [H00003014-M05] - Analysis of monoclonal antibody to H2AFX on HeLa cell. Antibody concentration 10 ug/ml

Applications for Histone H2AX Antibody (3F4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA.

Reviewed Applications

Read 1 review rated 4 using H00003014-M05 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Alternate Names

H2AFX

Entrez Gene IDs

3014 (Human)

Gene Symbol

H2AX

OMIM

601772 (Human)

UniProt

AAH04915 (Human)

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (3F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2AX Antibody (3F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Customer Reviews for Histone H2AX Antibody (3F4) - Azide and BSA Free (1)

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  • H2AX Antibody (3F4)
    Name: Anonymous
    Application: ImageStream
    Sample Tested: WI-38 human lung fibroblast cell line
    Species: Human
    Verified Customer | Posted 10/10/2016
    Histone H2AX Antibody (3F4) - Azide and BSA Free H00003014-M05

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Protocols

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FAQs

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