Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

Novus Biologicals | Catalog # NBP2-61155

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5245

Format

BSA Free
View all Histone H2AY Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from O75367, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

Western Blot: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Western Blot: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Western Blot: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunocytochemistry/ Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunocytochemistry/Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - Staining of CACO-2 cells using the Anti-H2AFY monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human tonsil shows strong nuclear immunoreactivity in lymphoid cells.
Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human kidney shows strong nuclear positivity in renal tubules cells.
Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.
Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human fallopian tube shows strong nuclear positivity in glandular and stromal cells.

Applications for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AY

macroH2A.1 (H2AFY) is 372 amino acids long, weighs approximately 40 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. macroH2a.1 has two shorter isoforms meeasuring 371 and 369 amino acids long, both weighing approximately 39 kDa. Current studies are being done on several diseases and disorders including medulloblastoma, lupus erythematosus, Huntington's disease, immunodeficiency, melanoma, and malaria. macroH2A.1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the chromatin regulation/ acetylation, systemic lupus erythematosus, and ATF-2 transcription factor pathways.

Long Name

H2A Histone Family, Member Y

Alternate Names

H2AF12M, H2AFJ, H2AFY, Histone MacroH2A1, Medulloblastoma Antigen MU-MB-50.205

Gene Symbol

MACROH2A1

Additional Histone H2AY Products

Product Documents for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

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Protocols

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