Histone H2B [ac Lys20] Antibody (GT578) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13538

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT578

Format

Azide and BSA Free
View all Histone H2B Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys20 of human Histone H2B. The exact sequence is proprietary.

Modification

ac Lys 20

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Histone H2B [ac Lys20] Antibody (GT578) - Azide and BSA Free

Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B (acetyl Lys20) antibody [GT578] detects Histone H2B (acetyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2B (acetyl Lys20) stained by Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Histone H2B [ac Lys20] Antibody (GT578)

Immunohistochemistry-Paraffin: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] -

Immunohistochemistry-Paraffin: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B antibody [GT578] detects Histone H2B protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H2B stained by Histone H2B antibody [GT578] (NBP3-13538) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Histone H2B [ac Lys20] Antibody (GT578) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents for Histone H2B [ac Lys20] Antibody (GT578) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2B [ac Lys20] Antibody (GT578) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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