Histone H2B, member S Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56347

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Drosophila, Mammal, Zebrafish

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H2B, member S Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSS of human Histone H2B; GenBank no. CAI19747.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B, member S Antibody - BSA Free

Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347]

Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0011.jpg
Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347]

Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347]

Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347] - Human heart stained with Histone H2B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347] - Analysis of Histone H2B using NB100-56347 at 1:250 in nuclear and cytoplasmic fractions made from Drosophila head extracts using Novus' Nuclear Extraction Kit. Data courtesy of Dr. Jerry Yin, University of Wisconsin-Madison.
Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347] - Top: Analysis of Histone H2B in human PBMC lysate with this antibody. Bottom: Western blot analysis of fractionated Cos-7 cells using this antibody anti-Histone H2B. Cos-7 cells were fractionated into nuclear cytosolic/membrane fractions. Approximately 15 ug of each fraction was separated on SDS PAGE. Lane 1) Nuclear fraction Lane 2) Cytosolic/membrane fraction.
Western Blot: Histone H2B, member S Antibody [NB100-56347]

Western Blot: Histone H2B, member S Antibody [NB100-56347]

Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0010.jpg
Histone H2B, member S Antibody

Western Blot: Histone H2B, member S Antibody [NB100-56347] -

BAY-87-2243 inhibits nuclear HIF-1 alpha protein expression. UT-SCC-5 tumors were treated either with BAY-87-2243 or carrier for 3, 5, or 7 days and were excised 24 h after end of treatment: a) immunohistochemistry, black arrows indicate the positive nuclear HIF-1 alpha staining, scale bar: 20 μm; b) western blots of nuclear HIF-1 alpha from different animals 3 days after BAY-87-2243 application; c) quantification of western blots by densitometry relative to histone-H2B, means ± SD.

Applications for Histone H2B, member S Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 28235947)

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

2 ug/ml
Application Notes
A 15 kDa band is observed. This antibody is routinely quality controlled in human lysates.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B, member S

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Humsn histone H2B is a 125 amino acid protein.

Alternate Names

H2B histone family S member10H2B/s, H2B histone family, member S, Histone H2B.s

Entrez Gene IDs

295278 (Rat)

Gene Symbol

H2BFS

Additional Histone H2B, member S Products

Product Documents for Histone H2B, member S Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Histone H2B, member S Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone H2B, member S Antibody - BSA Free

Customer Reviews for Histone H2B, member S Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Histone H2B, member S Antibody - BSA Free and earn rewards!

Have you used Histone H2B, member S Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies