Histone H3 [Trimethyl Lys27] Antibody

Novus Biologicals | Catalog # NBP2-16840

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Predicted:

Chimpanzee (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Dot Blot, Electron Microscopy

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.

Modification

Trimethyl Lys27

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3 [Trimethyl Lys27] Antibody

Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840]

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840]

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control).
Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A (tri-Methyl K27) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840]

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840]

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Hela xenograft, using Histon H3 (tri-Methyl K27) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Histone H3 [Trimethyl Lys27] Antibody

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Histone H3 [Trimethyl Lys27] Antibody

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Histone H3 [Trimethyl Lys27] Antibody

Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 stained by Histone H3 antibody (NBP2-16840) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Histone H3 [Trimethyl Lys27] Antibody

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Histone H3 [Trimethyl Lys27] Antibody

Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody (NBP2-16840) diluted at 1:2500.
Histone H3 [Trimethyl Lys27] Antibody

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody (NBP2-16840) diluted at 1:1000.
Histone H3 [Trimethyl Lys27] Antibody

Immunohistochemistry-Frozen: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (NBP2-16840) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594).
Blue: Nuclear staining with Hoechst 33342.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Histone H3 [Trimethyl Lys27] Antibody

Immunohistochemistry: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] -

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K27me3 (trimethyl Lys27) antibody (NBP2-16840) dilution: 1:100.

Applications for Histone H3 [Trimethyl Lys27] Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reported in scientific literature (PMID: 30143902)

Dot Blot

1:100 - 1:2000

Electron Microscopy

1:10 - 1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-16840 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A, H3K27Me3, H3pS28K27Me3

Gene Symbol

H3C14

Additional Histone H3 Products

Product Documents for Histone H3 [Trimethyl Lys27] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Histone H3 [Trimethyl Lys27] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
Interferons
Notch Pathway

Citations for Histone H3 [Trimethyl Lys27] Antibody

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-435 cell lysates
    Species: Human
    Verified Customer | Posted 06/13/2014
    Histone H3 [Trimethyl Lys27] Antibody NBP2-16840

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