Histone H3.1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-81964

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H3.1 Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around amino acids 8~12 (R-K-S-T-G).

Specificity

Detects endogenous levels of total Histone H3.1 protein. NB100-81964 is also cross-reactive with Histone H3.2 and H3.3 from human mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3.1 Antibody - BSA Free

Western Blot: Histone H3.1 Antibody [NB100-81964] -

Western Blot: Histone H3.1 Antibody [NB100-81964] - Analysis of extracts from 293 cells using NB100-81964
Histone H3.1 Antibody

Western Blot: Histone H3.1 Antibody [NB100-81964] -

Western Blot: Histone H3. 1 Antibody [NB100-81964] - Western blot analysis of extracts of various cell lines, using NB100-81964
Histone H3.1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody - BSA Free [NB100-81964] -

Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody - BSA Free [NB100-81964] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81964

Applications for Histone H3.1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.1

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Long Name

Histone Cluster 1 H3 Family Member A

Alternate Names

HIST1H3A

Entrez Gene IDs

8353 (Human); 319151 (Mouse)

Gene Symbol

H3C6

UniProt

P68431 (Human)

Additional Histone H3.1 Products

Product Documents for Histone H3.1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Histone H3.1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

Citations for Histone H3.1 Antibody - BSA Free

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Protocols

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