HIV-1 Tat specific factor 1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-03409
![Western Blot: HIV-1 Tat specific factor 1 AntibodyBSA Free [NBP3-03409]](https://resources.rndsystems.com/images/products/HIV-1-Tat-specific-factor-1-Antibody-Western-Blot-NBP3-03409-img0007.jpg "Western Blot: HIV-1 Tat specific factor 1 AntibodyBSA Free [NBP3-03409]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HIV-1 Tat specific factor 1 (NP_055315.2). MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HIV-1 Tat specific factor 1 Antibody - BSA Free
Western Blot: HIV-1 Tat specific factor 1 AntibodyBSA Free [NBP3-03409]
Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP3-03409] - Analysis of extracts of various cell lines, using HIV-1 Tat specific factor 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.![Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]](https://resources.rndsystems.com/images/products/HIV-1-Tat-specific-factor-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03409-img0009.jpg "Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]")
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP3-03409] - Paraffin-embedded human lung cancer using HIV-1 Tat specific factor 1 antibody at dilution of 1:100 (40x lens).![Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]](https://resources.rndsystems.com/images/products/HIV-1-Tat-specific-factor-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03409-img0005.jpg "Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]")
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP3-03409] - Paraffin-embedded mouse brain using HIV-1 Tat specific factor 1 antibody at dilution of 1:100 (40x lens).![Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]](https://resources.rndsystems.com/images/products/HIV-1-Tat-specific-factor-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03409-img0008.jpg "Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]")
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody - BSA Free [NBP3-03409]
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP3-03409] - Paraffin-embedded human stomach using HIV-1 Tat specific factor 1 antibody at dilution of 1:100 (40x lens).
Western Blot: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] -
Western Blot: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] - Western blot analysis of various lysates using HIV-1 Tat specific factor 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] -
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using HIV-1 Tat specific factor 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] -
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIV-1 Tat specific factor 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] -
Immunohistochemistry: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using HIV-1 Tat specific factor 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] -
Immunocytochemistry/ Immunofluorescence: HIV-1 Tat specific factor 1 Antibody - BSA Free [HIV-1 Tat specific factor 1] - Immunofluorescence analysis of C6 cells using HIV-1 Tat specific factor 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for HIV-1 Tat specific factor 1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HIV-1 Tat specific factor 1
Alternate Names
cofactor required for Tat activation of HIV-1 transcription, dJ196E23.2, HIV TAT specific factor 1, HIV Tat-specific factor 1, HIV-1 Tat specific factor 1, TAT-SF1
Gene Symbol
HTATSF1
Additional HIV-1 Tat specific factor 1 Products
Product Documents for HIV-1 Tat specific factor 1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIV-1 Tat specific factor 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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