HLA-ABCE Antibody (TP25.99SF) - BSA Free

Novus Biologicals | Catalog # NBP2-68006

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TP25.99SF

Format

BSA Free
View all HLA-ABCE Primary Antibodies »

Product Specifications

Immunogen

IFN-gamma-treated human melanoma cells COLO 38.

Specificity

The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for distinguishing between HLA-G and other HLA-class I antigens.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006]

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006]

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) [NBP2-68006] - Staining of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.
HLA-ABCE Antibody (TP25.99SF) - BSA Free

Western Blot: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006] -

Neonatal skin fibroblasts primed with inflammatory signals express less molecules that activate the immune system. (A) Schematic of the protocol used to stimulate neonatal (NSFs) and adult (ASFs) skin fibroblasts. (B) Western blots on ASF and NSF lysates for the detection of HLA-ABCE, HLA-DPDQDR, B7-H3 and alpha -tubulin (used as a loading control). (C) Densitometry quantifications normalized to alpha -tubulin. Statistics: Wald test with Tukey’s multiple-comparison tests; * p < 0.05, ** p < 0.01, *** p < 0.001, N = 6 different fibroblast populations (3 NSFs and 3 ASFs). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/13/6965), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Western Blot

1-2 µg/m
Application Notes
Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: HLA-ABCE

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Alternate Names

HLA-ABC, HLA-E, major histocompatibility complex, class I, A, major histocompatibility complex, class I, B, major histocompatibility complex, class I, C, major histocompatibility complex, class I, E, MHC class I

Additional HLA-ABCE Products

Product Documents for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLA-ABCE Antibody (TP25.99SF) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Customer Reviews for HLA-ABCE Antibody (TP25.99SF) - BSA Free

There are currently no reviews for this product. Be the first to review HLA-ABCE Antibody (TP25.99SF) - BSA Free and earn rewards!

Have you used HLA-ABCE Antibody (TP25.99SF) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies