HLA DPA Antibody

Novus Biologicals | Catalog # NBP2-16851

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HLA DPA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA DPA. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA DPA Antibody

HLA DPA Antibody

Western Blot: HLA DPA Antibody [NBP2-16851] -

Western Blot: HLA DPA Antibody [NBP2-16851] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HLA DPA antibody (NBP2-16851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HLA DPA Antibody [NBP2-16851]

Immunocytochemistry/ Immunofluorescence: HLA DPA Antibody [NBP2-16851]

Immunocytochemistry/Immunofluorescence: HLA DPA Antibody [NBP2-16851] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HLA DPA Antibody [NBP2-16851]

Immunohistochemistry-Paraffin: HLA DPA Antibody [NBP2-16851]

Immunohistochemistry-Paraffin: HLA DPA Antibody [NBP2-16851] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Applications for HLA DPA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DPA

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Alternate Names

DP alpha 1 chain, HLA class II histocompatibility antigen, DP alpha chain, HLADP, HLA-SB alpha chain, major histocompatibility complex, class II, DP alpha 1, MHC class II antigen, MHC class II DP3-alpha, MHC class II DPA1, MHC class II HLA-DPA1 antigen, Primed lymphocyte test-1

Gene Symbol

HLA-DPA1

UniProt

P20036 (Human)

Additional HLA DPA Products

Product Documents for HLA DPA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLA DPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HLA DPA Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA DPA Antibody

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  • Q: We are planning to buy the HLA-DPA1 and HLA-DMB Abs from your company to do the IHC in our Tumor samples. We saw the image of the tissue, and my PI wants to know if you can send us the exact protocol you have used to do the IHC for NBP2-16851 and NBP1-85326?

    A:

    These two antibodies should work with any general IHC-P protocol. Our general IHC-P protocol can be found here.

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