HLA DRA Antibody

Novus Biologicals | Catalog # NBP1-33458

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HLA DRA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA DRA. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%), Canine (87%).

Localization

Membrane |

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA DRA Antibody

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
HLA DRA Antibody

Western Blot: HLA DRA Antibody [NBP1-33458] -

Western Blot: HLA DRA Antibody [NBP1-33458] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DRA antibody [N2C3] (NBP1-33458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HLA DRA Antibody

Immunocytochemistry/ Immunofluorescence: HLA DRA Antibody [NBP1-33458] -

HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis.
Sample: Raji cells were fixed in 100% Methenol for 5 min.
Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (NBP1-33458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for HLA DRA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DRA

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Alternate Names

FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW

Gene Symbol

HLA-DRA

Additional HLA DRA Products

Product Documents for HLA DRA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HLA DRA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HLA DRA Antibody

There are currently no reviews for this product. Be the first to review HLA DRA Antibody and earn rewards!

Have you used HLA DRA Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies