HLA DRB1 Antibody (DA2) - BSA Free

Novus Biologicals | Catalog # NB100-2705

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DA2

Format

BSA Free
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Product Specifications

Immunogen

The membranes of LKT cells (a B lymphoblastoid cell line)

Epitope

1st domain, monomorphic.

Localization

Cell Membrane

Specificity

DA2 will detect dimers and the beta chain by western blot. DA2 will bind all DR and DP antigens except DR7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for HLA DRB1 Antibody (DA2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA-DRB1

This antibody can be used for HLA typing.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Entrez Gene IDs

3123 (Human)

Gene Symbol

HLA-DRB1

Additional HLA-DRB1 Products

Product Documents for HLA DRB1 Antibody (DA2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HLA DRB1 Antibody (DA2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA DRB1 Antibody (DA2) - BSA Free

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  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

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