HLA DRB5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-46107

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HLA DRB5 Primary Antibodies »

Product Specifications

Immunogen

major histocompatibility complex, class II, DR beta 5. (Uniprot# Q30154)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA DRB5 Antibody - BSA Free

HLA DRB5 Antibody - BSA Free

Western Blot: HLA DRB5 Antibody - BSA Free [NBP3-46107] -

Western Blot: HLA DRB5 Antibody - BSA Free [NBP3-46107] - HepG2 cells were subjected to SDS PAGE followed by western blot with (HLA DRB5 antibody) at dilution of 1:500
HLA DRB5 Antibody - BSA Free

Immunohistochemistry: HLA DRB5 Antibody - BSA Free [NBP3-46107] -

Immunohistochemistry: HLA DRB5 Antibody - BSA Free [NBP3-46107] - Immunohistochemistry of paraffin-embedded human tonsillitis using (HLA DRB5 antibody) at dilution of 1:50

Applications for HLA DRB5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA DRB5

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Alternate Names

DR beta-5, DR2-beta-2, DR9, DR-9, Dw2, FLJ76359, HLA class II histocompatibility antigen, DR beta 5 chain, HLA class II histocompatibility antigen, DR-5 beta chain, HLA class II histocompatibility antigen, DRB1-9 beta chain, HLA-DRB, human leucocyte antigen DRB5, leukocyte antigen class II, major histocompatibility complex, class II, DR beta 5, MHC class II antigen DRB1*9, MHC class II antigen DRB5, MHC class II HLA beta chain, MHC HLA-DR-beta cell surface glycoprotein, MHC HLA-DR-beta chain

Gene Symbol

HLA-DRB5

Additional HLA DRB5 Products

Product Documents for HLA DRB5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HLA DRB5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA DRB5 Antibody - BSA Free

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  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

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