HLTF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83256

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HLTF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLTF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HLTF Antibody [NBP1-83256]

Immunocytochemistry/ Immunofluorescence: HLTF Antibody [NBP1-83256]

Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Western Blot: HLTF Antibody [NBP1-83256]

Western Blot: HLTF Antibody [NBP1-83256]

Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
HLTF Antibody - BSA Free Western Blot: HLTF Antibody - BSA Free [NBP1-83256]

Western Blot: HLTF Antibody - BSA Free [NBP1-83256]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for HLTF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLTF

Helicase-like Transcription Factor (HLTF) is a member of the the SWI/SNF family of nuclear enzymes that remove proteins that coil around DNA. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. HLTF proteins aid the stabilization and regulation of the production of proteins in the cell. Inactivation of the HTLF gene may contribute to the transformation of normal colon cells into cancer cells.

Alternate Names

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC 3.6.1, EC 3.6.4.-, EC 6.3.2.-, helicase-like transcription factor, HIP116ARING finger protein 80, HIP116matrix associated, actin dependent regulator of chromatin, RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3, subfamily a, member 3, Sucrose nonfermenting protein 2-like 3, ZBU1

Entrez Gene IDs

6596 (Human)

Gene Symbol

HLTF

UniProt

Q14527 (Human)

Additional HLTF Products

Product Documents for HLTF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLTF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HLTF Antibody - BSA Free

Customer Reviews for HLTF Antibody - BSA Free

There are currently no reviews for this product. Be the first to review HLTF Antibody - BSA Free and earn rewards!

Have you used HLTF Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies