HLTF Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16857

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HLTF Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLTF Antibody - BSA Free

Western Blot: HLTF Antibody [NBP2-16857]

Western Blot: HLTF Antibody [NBP2-16857]

Western Blot: HLTF Antibody [NBP2-16857] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: HLTF Antibody [NBP2-16857]

Immunocytochemistry/ Immunofluorescence: HLTF Antibody [NBP2-16857]

Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP2-16857] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP2-16857]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP2-16857]

Immunohistochemistry-Paraffin: HLTF Antibody [NBP2-16857] - Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: HLTF Antibody [NBP2-16857]

Immunoprecipitation: HLTF Antibody [NBP2-16857]

Immunoprecipitation: HLTF Antibody [NBP2-16857] - HLTF antibody immunoprecipitates HLTF protein in IP experiments. IP Sample: HeLa whole cell lysate/extract. A. 40 ug HeLa whole cell lysate/extract. B. Control with 2 ug of preimmune rabbit IgG. C. Immunoprecipitation of HLTF protein by 2 ug of HLTF antibody. 7.5% SDS-PAGE. The immunoprecipitated HLTF protein was detected by HLTF antibody diluted at 1:1000.

Applications for HLTF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLTF

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq]

Alternate Names

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC 3.6.1, EC 3.6.4.-, EC 6.3.2.-, helicase-like transcription factor, HIP116ARING finger protein 80, HIP116matrix associated, actin dependent regulator of chromatin, RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3, subfamily a, member 3, Sucrose nonfermenting protein 2-like 3, ZBU1

Gene Symbol

HLTF

UniProt

Q14527 (Human)

Additional HLTF Products

Product Documents for HLTF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLTF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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