Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F4

Format

Azide and BSA Free
View all HMBS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HMBS (NP_000181) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMBS Antibody (OTI3F4) - Azide and BSA Free

Western Blot: HMBS Antibody (OTI3F4)Azide and BSA Free [NBP2-70894]

Western Blot: HMBS Antibody (OTI3F4)Azide and BSA Free [NBP2-70894]

Western Blot: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS.
Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of Human Kidney tissue.
Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894]

Immunohistochemistry: HMBS Antibody (OTI3F4) - Azide and BSA Free [NBP2-70894] - Analysis of Human lymphoma tissue.

Applications for HMBS Antibody (OTI3F4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HMBS

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

hydroxymethylbilane synthasePre-uroporphyrinogen synthase, PBGDPBG-D, PORC, porphobilinogen deaminase, porphyria, acute; Chester type, UPSEC 2.5.1.61, uroporphyrinogen I synthase, uroporphyrinogen I synthetase

Gene Symbol

HMBS

Additional HMBS Products

Product Documents for HMBS Antibody (OTI3F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HMBS Antibody (OTI3F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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