HMGB1/HMG-1 [ac Lys29] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-15486

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HMGB1/HMG-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic acetylated peptide around K29 of human HMGB1.

Modification

ac Lys29

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGB1/HMG-1 [ac Lys29] Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of C6 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for HMGB1/HMG-1 [ac Lys29] Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 [ac Lys29] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 [ac Lys29] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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