![HMGB4 Antibody (OTI5G3) Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44707_mouse-hmgb4-mab-oti5g3-azide-and-bsa-free-immunohistochemistry-7520262256152.jpg "Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5G3
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Product Specifications
Immunogen
Full length human recombinant protein of human HMGB4(NP_001008728) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HMGB4 Antibody (OTI5G3)
Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HMGB4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![HMGB4 Antibody (OTI5G3) Western Blot;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44707_mouse-hmgb4-mab-oti5g3-azide-and-bsa-free-western-blot-7520262255401.jpg "Western Blot;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Western Blot;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY HMGB4 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMGB4 (1:2000). Positive lysates![HMGB4 Antibody (OTI5G3) Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44707_mouse-hmgb4-mab-oti5g3-azide-and-bsa-free-immunohistochemistry-7520262259178.jpg "Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HMGB4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![HMGB4 Antibody (OTI5G3) Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44707_mouse-hmgb4-mab-oti5g3-azide-and-bsa-free-immunohistochemistry-7520262244338.jpg "Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HMGB4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![HMGB4 Antibody (OTI5G3) Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44638_mouse-pepd-mab-oti5d5-azide-and-bsa-free-immunohistochemistry-75202622435217.jpg "Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HMGB4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![HMGB4 Antibody (OTI5G3) Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-](https://resources.rndsystems.com/images/products/nbp3-44707_mouse-hmgb4-mab-oti5g3-azide-and-bsa-free-immunohistochemistry-75202622471116.jpg "Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-")
Immunohistochemistry;HMGB4 Antibody (OTI5G3)[NBP3-44707]-
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HMGB4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.Applications for HMGB4 Antibody (OTI5G3)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
>95%
Reconstitution
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized powder ( 1X PBS, pH 7.3,)
Preservative
8% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Calculators
Background: HMGB4
Alternate Names
dJ1007G16.5, FLJ40388, high mobility group box 4, high mobility group protein B4, HMG2 like, MGC88128
Gene Symbol
HMGB4
Additional HMGB4 Products
Product Documents for HMGB4 Antibody (OTI5G3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HMGB4 Antibody (OTI5G3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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