HMGN1/HMG14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47433

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HMGN1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QAEVANQETKEDLPAENGETKTEESPASDEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGN1/HMG14 Antibody - BSA Free

Western Blot: HMGN1/HMG14 Antibody [NBP2-47433]

Western Blot: HMGN1/HMG14 Antibody [NBP2-47433]

Western Blot: HMGN1/HMG14 Antibody [NBP2-47433] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2
Immunocytochemistry/ Immunofluorescence: HMGN1/HMG14 Antibody [NBP2-47433]

Immunocytochemistry/ Immunofluorescence: HMGN1/HMG14 Antibody [NBP2-47433]

Immunocytochemistry/Immunofluorescence: HMGN1/HMG14 Antibody [NBP2-47433] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP2-47433]

Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP2-47433]

Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP2-47433] - Staining of human rectum shows moderate nuclear positivity in glandular cells.

Applications for HMGN1/HMG14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGN1

HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box;" that of the HMGN family, the "nucleosomal binding domain;" and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."

Long Name

High Mobility Group Nucleosome Binding Domain 1

Alternate Names

HMG14

Gene Symbol

HMGN1

Additional HMGN1 Products

Product Documents for HMGN1/HMG14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HMGN1/HMG14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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