![Western Blot: HN1 Antibody [NBP1-32510]](https://resources.rndsystems.com/images/products/HN1-Antibody-Western-Blot-NBP1-32510-img0007.jpg "Western Blot: HN1 Antibody [NBP1-32510]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Monkey (100%). Backed by our 100% Guarantee.
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human HN1. The exact sequence is proprietary.
Reactivity Notes
Mouse (88%), Rat (82%), Bovine (88%), Dog (83%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HN1 Antibody - BSA Free
Western Blot: HN1 Antibody [NBP1-32510]
Western Blot: HN1 Antibody [NBP1-32510] - Wild-type (WT) and HN1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![ELISA: HN1 Antibody [NBP1-32510]](https://resources.rndsystems.com/images/products/HN1-Antibody-ELISA-NBP1-32510-img0005.jpg "ELISA: HN1 Antibody [NBP1-32510]")
ELISA: HN1 Antibody [NBP1-32510]
ELISA: HN1 Antibody [NBP1-32510] - An ELISA plate is coated with 50 uL of HN1 recombinant protein at concentration ranged from 0.004 ug/mL to 1 ug/mL. The coated protein is detected with HN1 antibody at 0.25 ug/mL.![ELISA: HN1 Antibody [NBP1-32510]](https://resources.rndsystems.com/images/products/HN1-Antibody-ELISA-NBP1-32510-img0006.jpg "ELISA: HN1 Antibody [NBP1-32510]")
ELISA: HN1 Antibody [NBP1-32510]
ELISA: HN1 Antibody [NBP1-32510] - An ELISA plate is coated with 50 uL of HN1 recombinant protein at concentration ranged from 0.004 ug/mL to 1 ug/mL. The coated protein is detected with HN1 antibody at 0.25 ug/mL.
Western Blot: HN1 Antibody [NBP1-32510] -
Western Blot: HN1 Antibody [NBP1-32510] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody (NBP1-32510) diluted at 1:3000.
Western Blot: Rabbit Polyclonal HN1 Antibody [NBP1-32510] -
Western Blot: Rabbit Polyclonal HN1 Antibody [NBP1-32510] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELISA: HN1 Antibody [NBP1-32510] -
An ELISA plate is coated with 50 uL of HN1 recombinant protein at concentration ranged from 0.004 ug/mL to 1 ug/mL. The coated protein is detected with HN1 antibody (NBP1-32510) at 0.25 ug/mL.Applications for HN1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:10000
Western Blot
1:500-1:3000
Application Notes
IHC-P Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HN1
Alternate Names
Androgen-regulated protein 2, ARM2hematological and neurological expressed 1 protein, hematological and neurological expressed 1, HN1A, OPMRX60
Gene Symbol
JPT1
Additional HN1 Products
Product Documents for HN1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HN1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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