HNF-3 alpha/FoxA1 Antibody (23GB1170)

Novus Biologicals | Catalog # NBP3-51979

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1170
View all HNF-3 alpha/FoxA1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HNF-3 alpha/FoxA1 (Uniprot: P55317)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (23GB1170)

HNF-3 alpha/FoxA1 Antibody (23GB1170)

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (23GB1170)[NBP3-51979] -

Immunocytochemical staining of HepG2 cells with HNF-3 alpha/FoxA1 antibody (1:1,000). Nuclei were stained blue with DAPI; HNF-3 alpha/FoxA1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
HNF-3 alpha/FoxA1 Antibody (23GB1170)

Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (23GB1170)[NBP3-51979] -

Validation of HNF-3 alpha/FoxA1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with HNF-3 alpha/FoxA1 antibody (1:2,000) and analyzed using BD flow cytometer.
HNF-3 alpha/FoxA1 Antibody (23GB1170)

Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (23GB1170)[NBP3-51979] -

Flow cytometric analysis of HNF-3 alpha/FoxA1expression in HepG2 cells using HNF-3 alpha/FoxA1 antibody (1:2,000). Green, isotype control; red, forkhead box A1.
HNF-3 alpha/FoxA1 Antibody (23GB1170)

Western Blot: HNF-3 alpha/FoxA1 Antibody (23GB1170)[NBP3-51979] -

Western blotting analysis using HNF-3 alpha/FoxA1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for HNF-3 alpha/FoxA1 Antibody (23GB1170)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (23GB1170)

Certificate of Analysis

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (23GB1170)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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