hnRNP H Antibody - BSA Free

Novus Biologicals | Catalog # NB100-385

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP H Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hnRNP H Antibody - BSA Free

Western Blot: hnRNP H Antibody [NB100-385]

Western Blot: hnRNP H Antibody [NB100-385]

Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP H antibody NB100-385 (lot 2) used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. hnRNP H was also immunoprecipitated by a prior lot (lot 1) of this antibody. Detection: Chemiluminescence with exposure times 30 second (A and B).
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385]

Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385]

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NB100-385] - analysis of hnRNP H in SH-SY5Y cells using anti-hnRNP H antibody (green). Cells were fixed in 4% PFA in PBS for 15 min at room temperature. Image from verified customer review.
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385]

Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385]

Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Simple Western: hnRNP H Antibody [NB100-385]

Simple Western: hnRNP H Antibody [NB100-385]

Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
hnRNP H Antibody - BSA Free

Western Blot: hnRNP H Antibody - BSA Free [NB100-385] -

Knockdown of HNRNPH1 leads to decreased CDK2/4/6 protein levels and elevated apoptosis markers.a Immunoblot analysis of whole-cell lysates prepared from RMS cells (RD, RH30, and RH41) 2 and 4 days post-HNRNPH1 siRNA transfection with antibodies against CDK2/4/6, HNRNPH1, and beta -actin. b Immunoblot analysis of whole-cell lysates prepared from RMS cells (RD, RH30, and RH41) 4 and 7 days post-HNRNPH1 siRNA transfection with antibodies against PARP, cleaved PARP, caspase-3, cleaved caspase-3, HNRNPH1, and beta -actin. All quantification is shown below the gels and normalized to actin. CDK4 was quantified using the short exposure for RH30 cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29362363), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for hnRNP H Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

4-10 ug/mg of lysate

Simple Western

1:1000

Western Blot

1:10000-1:30000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 58 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. hnRNP H antibody validated for ICC/IFfrom a verified customer review.

Reviewed Applications

Read 1 review rated 3 using NB100-385 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: hnRNP H

The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.

Alternate Names

DKFZp686A15170, heterogeneous nuclear ribonucleoprotein H, heterogeneous nuclear ribonucleoprotein H1 (H), hnRNP H, HNRPH, HNRPH1hnRNPH

Entrez Gene IDs

3187 (Human)

Gene Symbol

HNRNPH1

UniProt

NP_005511.1 (Human)

Additional hnRNP H Products

Product Documents for hnRNP H Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for hnRNP H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP H Antibody - BSA Free

Customer Reviews for hnRNP H Antibody - BSA Free (1)

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  • Name: Simona Darovic
    Application: Immunofluorescence
    Sample Tested: SH-SY5Y cell culture
    Species: Human
    Verified Customer | Posted 01/21/2015
    hnRNP H antibody
    hnRNP H Antibody - BSA Free NB100-385

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