hnRNP K Antibody

Novus Biologicals | Catalog # NBP2-24532

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Opossum, Primate

Predicted:

Chimpanzee (100%), Equine (93%), Monkey (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all hnRNP K Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acid 200 to 250 of human hnRNPK was used as immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP K Antibody

Western Blot: hnRNP K Antibody [NBP2-24532]

Western Blot: hnRNP K Antibody [NBP2-24532]

Western Blot: hnRNP K Antibody [NBP2-24532] - analysis of hnRNPK in K562 cell lysate in the 1) absence and2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532]

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532]

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532]

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532]

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.
Simple Western: hnRNP K Antibody [NBP2-24532]

Simple Western: hnRNP K Antibody [NBP2-24532]

Simple Western: hnRNP K Antibody [NBP2-24532] - Simple Western lane view shows a specific band for hnRNP K in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for hnRNP K Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Simple Western

1:50

Western Blot

2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 61 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP K

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Alternate Names

dC-stretch binding protein, FLJ41122, heterogeneous nuclear ribonucleoprotein K, hnRNP K, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNPHNRPKCSBP

Gene Symbol

HNRNPK

Additional hnRNP K Products

Product Documents for hnRNP K Antibody

Certificate of Analysis

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Product Specific Notices for hnRNP K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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