Hoechst 33258
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98%
Biological Activity
Hoechst 33258 is a fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.Optical Data for Hoechst 33258
| λabs | 352 nm |
|---|---|
| λem | 461 nm |
| Application | Flow Cytometry |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation.
Oberhammer et al.
EMBO J., 1993;12:3679 -
Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex.
Jin and Breslauer
Proc.Natl.Acad.Sci.U.S.A., 1988;85:8939
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Citations for Hoechst 33258
The citations listed below are publications that use Tocris products. Selected citations for Hoechst 33258 include:
2 Citations: Showing 1 - 2
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Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity.
Authors: Moreno Et al.
Life Sci Alliance 2019;2
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CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro.
Authors: Terheyden-Keighley Et al.
Biol Open 2018;7
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