Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free

Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]

Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.

Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free [NBP3-05459] -

Ablation of ASC in stromal vascular fractions (SVF) cells improved the lipogenesis but impaired lipolysis. (a–d) Primary SAT SVF cells of LM and Asc−/− mice (Red-LM mice, Blue-Asc−/− mice). (a) Oil Red O staining of the 6th day under lipogenesis inducement treated primary SAT SVF cells, separated from LM and Asc−/− mice, scale bar 200 um. (b) The expression of lipogenesis genes was analyzed by RT-qPCR. (c) Expression of lipogenesis (ACC, FASN, FABP4) and lipolysis (p-HSL, HSL, ATGL) protein in indicated cells. (d–h) Primary SAT SVF cells separated from WT mice were transfected with siRNA targeting for ASC on the 4th day (Blue-NC, Yellow-si Asc). (d,e) mRNA and protein level of ASC in indicated cells. (f) Oil Red O staining of the 6th day under lipogenesis inducement treated in indicated cells, scale bar 200 um. (g) Expression of lipogenesis genes in indicated cells. (h) Expression of lipogenesis (ACC, FASN, FABP4) and lipolysis (p-HSL, HSL, ATGL) protein in indicated cells. Data are presented as means +/- SD from three independent experiments. * p < 0.05 and ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36077447), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free [NBP3-05459] -

Ablation of ASC in stromal vascular fractions (SVF) cells improved the lipogenesis but impaired lipolysis. (a–d) Primary SAT SVF cells of LM and Asc−/− mice (Red-LM mice, Blue-Asc−/− mice). (a) Oil Red O staining of the 6th day under lipogenesis inducement treated primary SAT SVF cells, separated from LM and Asc−/− mice, scale bar 200 um. (b) The expression of lipogenesis genes was analyzed by RT-qPCR. (c) Expression of lipogenesis (ACC, FASN, FABP4) and lipolysis (p-HSL, HSL, ATGL) protein in indicated cells. (d–h) Primary SAT SVF cells separated from WT mice were transfected with siRNA targeting for ASC on the 4th day (Blue-NC, Yellow-si Asc). (d,e) mRNA and protein level of ASC in indicated cells. (f) Oil Red O staining of the 6th day under lipogenesis inducement treated in indicated cells, scale bar 200 um. (g) Expression of lipogenesis genes in indicated cells. (h) Expression of lipogenesis (ACC, FASN, FABP4) and lipolysis (p-HSL, HSL, ATGL) protein in indicated cells. Data are presented as means +/- SD from three independent experiments. * p < 0.05 and ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36077447), licensed under a CC-BY license. Not internally tested by Novus Biologicals.