HOXA11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80228

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HOXA11 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide directed towards the C terminal of human HOXA11. Peptide sequence KERRRRPESSSSPESSSGHTEDKAGGSGGQRTRKKRCPYTKYQIRELERE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31665361).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for HOXA11 Antibody - BSA Free

Western Blot: HOXA11 Antibody [NBP1-80228]

Western Blot: HOXA11 Antibody [NBP1-80228]

Western Blot: HOXA11 Antibody [NBP1-80228] - Titration: 0.2-1 ug/ml, Positive Control: Human Uterus.
Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228]

Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228]

Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228] - Human Uterus Tissue, 4-8ug/ml.
HOXA11 Antibody - BSA Free

Western Blot: HOXA11 Antibody - BSA Free [NBP1-80228] -

BM transplantation from WT donors leads to decidualization reaction in Hoxa11-null mice.(A-H) Sections of E5.5 implantation sites from WTWT BMT, KOWT BMT, and KOKO BMT stained with HE (A and B), CD31 (C and D), PR (E and F), and PCNA (G and H). B, D, F, and H are higher magnification photomicrographs of A, C, E, and G, respectively. Black arrows point to embryos. (J, K and L) Quantification of pecent PR-positive cells (J), percent PCNA-positive cells (K), and mean blood vessel luminal area (μm2) in the endometrium (n = 3–4 mice/group). (I) Sections of E5.5 implantation sites from WTWT BMT, KOWT BMT, and KOKO BMT co-stained with Hoxa11 (green), CD45 (red), and counterstained with DAPI (blue). Arrows point to Hoxa11-expressing cells. (M) Relative uterine mRNA expression of HoxA11 and implantation-related genes Prl8a2, Lif, and Msx1 normalized to GAPDH in WTWT BMT versus KOWT BMT versus KOKO BMT on E5.5 (n = 4–5 mice/group). (N) Uterine Hoxa11 protein expression in KOWT BMT versus KOKO BMT on E5.5. Bar graphs represent mean +/- SEM. p-values are noted on the graphs. Scale bars, 100 μm (A, C, E, G), 50 μm (B, D, F, H). Underlying data are available in S1 Data. BM, bone marrow; BMT, BM transplant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HE, hematoxylin–eosin; Hoxa11, Homeobox a11; KO, knockout; PCNA, proliferating cell nuclear antigen; PR, progesterone receptor; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31513564), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HOXA11 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-80228 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOXA11

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia.

Alternate Names

homeo box A11, homeobox A11, Homeobox protein Hox-1I, homeobox protein HOXA11, homeobox protein Hox-A11, HOX1, HOX1Ihomeo box 1I

Gene Symbol

HOXA11

UniProt

NP_034580 (Mouse)

Additional HOXA11 Products

Product Documents for HOXA11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HOXA11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HOXA11 Antibody - BSA Free

Customer Reviews for HOXA11 Antibody - BSA Free (1)

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  • Name: Ashwini Katre
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human pap serous carcinoma
    Species: Human
    Verified Customer | Posted 03/05/2014
    Human pap serous carcinoma stained for HOXA11
    HOXA11 Antibody - BSA Free NBP1-80228

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Protocols

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