HOXA5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83235

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HOXA5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MHSGRYGYGYNGMDLSVGRSGSGHFGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQSEPSPAPPAQPQIYPWMRKLHISHDN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HOXA5 Antibody - BSA Free

HOXA5 Antibody

IHC-P - HOXA5 Antibody [NB-P1-83235] -

IHC-P - HOXA5 Antibody [NB-P1-83235] -Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
HOXA5 Antibody

IHC-P-HOXA5 Antibody [NBP1-83235] -

IHC-P-HOXA5 Antibody [NBP1-83235] -Staining of human endometrium shows moderate nuclear positivity in glandular cells.
HOXA5 Antibody

IHC-P-HOXA5 Antibody [NBP1-83235] -

IHC-P-HOXA5 Antibody [NBP1-83235] -
HOXA5 Antibody

IHC-P-HOXA5 Antibody [NBP1-83235] -

IHC-P-HOXA5 Antibody [NBP1-83235] -Staining of human testis shows strong nuclear positivity in seminiferous ducts.
HOXA5 Antibody - BSA Free Western Blot: HOXA5 Antibody - BSA Free [NBP1-83235]

Western Blot: HOXA5 Antibody - BSA Free [NBP1-83235]

Analysis in control (vector only transfected HEK293T lysate) and HOXA5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
HOXA5 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: HOXA5 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: HOXA5 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-HOXA5 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for HOXA5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOXA5

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis. [provided by RefSeq]

Alternate Names

homeo box 1C, homeo box A5, homeobox A5, Homeobox protein Hox-1C, homeobox protein HOXA5, homeobox protein Hox-A5, HOX1, HOX1.3, HOX1CMGC9376

Gene Symbol

HOXA5

Additional HOXA5 Products

Product Documents for HOXA5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HOXA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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